In actual operation, the operation and maintenance of the high-speed refrigerated centrifuge should be standardized to ensure the normal operation of the instrument, and at the same time, the safety of the staff can also be guaranteed.
1. Turn on the centrifuge switch and enter the standby state.
2. Choose a suitable rotor: during centrifugation, the liquid contained in the centrifuge tube cannot exceed 2/3 of the total volume, otherwise the liquid is easy
For overflow; before and after use, pay attention to whether there is any liquid residue in the rotor, and keep it dry. Convert
When turning the head, pay attention to make the bayonet of the centrifuge shaft and the head locked firmly.
2. The balance error of the centrifuge tube should be within 0.1 g.
3. Select the centrifugal parameters:
a) Press the temperature setting button, then use the numeric keys to set the centrifugal temperature, and press Enter to confirm.
b) Press the speed setting button to switch between RPM / RCF setting files, use the numeric keys to set the centrifugal speed,
Enter to confirm.
c) Press the rotor setting button, then use the numeric keys to set the rotor type, and press Enter to confirm.
d) Press the time setting button, then use the numeric keys to set the centrifugal time, and press Enter to confirm.
e) The brake or acceleration speed of the centrifuge is generally set between 0 and 4, and it should not be adjusted frequently.
4. Put the balanced centrifuge tube symmetrically into the rotor. Close the cover and tighten the screws.
5. Press the door of the centrifuge. If the door is not closed firmly, the centrifuge will not start.
6. Press the START button to start centrifugation. After centrifugation starts, wait for the centrifugal speed to reach the set speed before
Open, once the centrifuge is found to be abnormal (such as imbalance causing the machine to vibrate significantly, or the noise is very loud),
Should immediately press the STOP button, if necessary, directly press the power switch to cut off the power, stop centrifugation, and find out the cause.
7. If the machine finds a fault, please contact the relevant personnel in time.
8. After use, please clean the rotor and centrifuge cavity, do not close the centrifuge cover, which is conducive to moisture evaporation.
9. After use, you must register and indicate the use.
Precautions
1. When the centrifuge is in the pre-cooling state, the centrifuge cover must be closed. After the centrifuge is completed, the rotor should be taken out and placed upside down on the experimental table. The remaining water in the chamber should be dried, and the centrifuge cover is open.
2. During ultracentrifugation, the liquid must be filled with the centrifuge tube, and vacuum should be evacuated when it is separated from the centrifuge tube. If the sealing of the centrifuge tube is poor, the liquid cannot be filled up to prevent overflow, which will affect the normal operation of the sensor.
3. When the rotor is pre-cooled, the rotor cover can be placed on the platform of the centrifuge, or placed on the experimental table, and it must not be tightened and floated on the rotor, because the rotor cover will be Will fly out and cause an accident!
4. After tightening the rotor cover, be sure to touch the seamless gap between the rotor and the cover with your fingers. If there is a gap, unscrew and re-tighten the centrifuge until the seamless gap is confirmed.
5. Be sure to ground the wire when using. The material added in the centrifuge tube should be relatively balanced. If it causes imbalance on both sides, it will cause great damage to the centrifuge and at least shorten the service life of the centrifuge.
6. During the centrifugation process, the operator must not leave the centrifuge room. Once an abnormal situation occurs, the operator cannot turn off the power (POWER) and must press STOP. Fill in the centrifuge use record before pre-cooling.
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