In the two-way agar diffusion experiment, the antigen and corresponding antibody are added to adjacent wells in the same gel plate. The two diffuse with each other, and when they reach the location where their concentration ratio is appropriate, a precipitate of antigen-antibody complex will form.
2. Operation steps
1. Take a clean glass plate, rinse with a small amount of 75% ethanol, dry it on a water platform, melt 1% agarose, and keep it warm in a 56 ° C water bath.
2. Spread the agarose on the glass plate with a thickness of about 1㎜.
3. Punch, the hole distance is 4㎜,
4. Dilute the antiserum to different concentrations of 1: 2, 1: 4, 1: 8, 1:16, 1:32 by the double dilution method.
Antibodies of different concentrations are added to the surrounding wells, and antigen is added to the central well.
Place the gel plate in a wet box at room temperature overnight. Observe the results. 3. The experimental results have different concentrations and form different precipitated lines.
Safety Razor For Men,Razor For Private Area Male,Shaving Trimmer Machine,Electric Shaver Argos
Ningbo Chuangxiang Electrical Appliance Co.,Ltd , https://www.hshaircurler.com