With the continuous development of biomedical imaging technology, near infrared fluorescence imaging technology has received more and more attention and application in the field of biomedical research. Among them, near-infrared two-region (1000 nm-1400 nm) fluorescence has strong penetrability to biological tissues and high imaging signal-to-noise ratio. This area of ​​fluorescence imaging technology has shown great potential in the field of biological imaging. Quantum dots (Quantum dots, QDs) as a new type of nano-fluorescent probes have the unparalleled advantages of traditional fluorescent dyes such as high brightness, strong light stability, and adjustable spectrum. They are obtained in biomarkers, imaging and sensing It has been widely used, and the development of QDs with near-infrared two-region fluorescence emission, good biocompatibility, and high quantum yield is currently an important challenge for its in vivo fluorescence imaging.
On the basis of "Single Source Precursor Preparation of Ag2S Near-Infrared Quantum Dots" (J. Am. Chem. Soc. 2010, 132, 1470–1471), Wang Qiangbin's research group of the Suzhou Institute of Nanotechnology and Nanobionics, Chinese Academy of Sciences Ag2S near-infrared QDs with higher quantum yield, better biocompatibility, and uniform controllable size were prepared. Through cooperation with the research group of Professor Dai Hongjie of Stanford University in the United States, cell imaging and toxicity studies were conducted using Ag2S QDs. The results show that the modification of different biorecognition molecules on the surface of water-soluble Ag2S QDs can achieve specific labeling to different cell lines, and the Ag2S QDs have almost no cytotoxicity (ACS Nano 2012, 6, 3695–3702).
On the basis of the above work, Wang Qiangbin's research group and Professor Dai Hongjie's research group continue to cooperate and further use Ag2S QDs for animal in vivo imaging research. The results show that due to the high permeability and retention effect of tumor tissue on macromolecules (referred to as the EPR effect), the tumor has a high uptake of QDs (Figure 2), which provides an important role for early diagnosis of tumors and visualization of surgery. technical foundation. At the same time, they tracked the fate of QDs introduced into mice, and found that except for the QDs enriched in tumor sites, most of the other QDs were continuously excreted with feces and urine after 24 hours of injection; after a week, various organs (Except liver and spleen) QDs have been basically eliminated (Figure 3).
The work has been published in the internationally renowned magazine Angewandte Chemie International Edition. Long-term in vivo metabolism, distribution, and toxicology studies of Ag2S QDs are ongoing.
This work was strongly supported by the "100-person plan" of the Chinese Academy of Sciences, the pilot project of the Chinese Academy of Sciences, the National Natural Science Foundation of China and the Ministry of Science and Technology.
Figure 1: (a) Ag2S QDs imaging diagram, (b) and (c) are the actual and dark field fluorescence photos of Ag2S QDs, (d) and (e) are the absorption and fluorescence spectra, (f) is TEM photo of Ag2S QDs.
Figure 2: Efficient uptake of Ag2S QDs by 4T1 tumors
Figure 3: Ag2S QDs in vivo retention and excretion
Charcoal Grill,Charcoal Bbq Grill,Small Charcoal Grill,Old Smokey Grill
NingBo AoYue Technology Co., Ltd. , https://www.aoyue-tech.com