Serum refers to the light yellow transparent liquid separated from fibrin by removing blood fibrin after blood coagulation or plasma to which fibrin has been removed. Its main role is to provide basic nutrients, provide hormones and various growth factors, provide binding proteins, provide contact promotion and extension factors to protect the cells from mechanical damage and play a certain role in protecting cells in culture. When we save the serum, we need to pay attention to the following points in order to successfully complete your experiment: 1. The thawed bottled serum needs to be gradually thawed: -20 ℃ to -70 ℃ The serum in the low temperature refrigerator is placed in the 4 ℃ refrigerator to dissolve 1 Day. Then move to room temperature and dispense after all are dissolved. During the dissolution process, you need to gently shake it evenly (be careful not to cause bubbles) to make the temperature and ingredients uniform and reduce the occurrence of precipitation. Do not directly remove the serum from -20 Enter thawing at 37 ℃, so that the temperature changes too much, and it is easy to cause protein aggregation and precipitation. 2. Precipitate floc in serum: mainly caused by denaturation of lipoprotein in serum and fibrin in serum after thawing, these The floc will not affect the quality of the serum itself. It can be removed by centrifugation at 3000 rpm for 5 minutes or without treatment. "Little black spots" under the microscope: after heat-treated serum, the formation of precipitates will increase significantly. 3. Heat extinction Live refers to heating the serum that has been completely thawed at 56 ° C for 30 minutes. It must be shaken regularly during the heating process. The purpose of this heat treatment is to inactivate the complement components in the serum. Unless necessary, this heat is generally not recommended. Treatment, because heat treatment will cause a significant increase in serum precipitates, and it will also affect the quality of serum. Complements are involved in the reaction: cytotoxicity, smooth muscle cell contraction, mast cells and platelets release histamine, enhance phagocytosis, promote lymphocytes and giant cells Phage cells undergo chemotactic and activation. 4. Serum that needs long-term storage must be stored in a -20 ℃-70 ℃ low temperature refrigerator. Do not store in a 4 ℃ refrigerator for more than 1 month. The volume will increase when the serum freezes About 10%, therefore, before the serum is frozen into the low temperature refrigerator, a certain volume of space must be reserved, otherwise it is easy to cause pollution or freeze cracking of the glass bottle.
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