Manual of human dopamine kit This reagent is for research use only Specimen: Serum 1. Reagent composition 1. Precision microplate Microtest Plates 96 wells 2. Enzyme Conjugate 12.0ml 3, Standard 5 x 1.0ml 4. Color Reagent A, Substrate A 6.0ml5, Color Reagent B, Substrate B 6.0ml 6, Stop Solution 6.0ml 7, Concentrated Wash Solution (1:20) Rinsing Buffer 60.0ml 8, 5 times sample dilution dilution 15.0m 2. Precautions This reagent is an in vitro test reagent. It is used within the validity period. The reagent should be regarded as an infectious agent. Reagents with different batch numbers cannot be mixed. Before use, the reagents in the box should be taken out and left at room temperature for at least 30 minutes. Store at 2-8 ° C, do not freeze, please see the label in the box for the expiration date. After the concentrated washing solution crystallizes, please incubate at 37 ° C for 15 minutes. 3. Operation steps Add 100 ul of the diluted sample and standard respectively to each well. The plate was incubated at 37 ° C for 30 minutes. Shake off the liquid in the plate, wash with the application washing liquid, stay for 30 seconds each time, pat dry on the paper. Repeat the operation 5 times. Add 100ul of enzyme-labeled coupling solution to each well. The plate was incubated at 37 ° C for 30 minutes. Shake off the liquid in the plate, wash with the application washing liquid, stay for 30 seconds each time, pat dry on the paper. Repeat the operation 5 times. Add 50 ul each of Substrate A and Substrate B to each well, and place in a 37 ° C incubator for 15 minutes. 8. Add 50ul of stop solution to each well and read the OD value at 450nm. 4. Result judgment instrument value: Read the OD value of each well on a microplate reader with a wavelength of 450nm. Detection value range: 0-400nmol / L Sensitivity: 1.0 nmol / L
Macrame Cushions
Our Macrame Cushions are handmade . Diy macrame pillow , the combination of colors makes it an ideal accent pillow for any decor . New beautiful pillow cover made in hand tied macrame . Very nice and beautiful stylish with the fringe giving it a light and airy feel .
They are made of high quality cotton linen materials , without odors or allergens retardants , mercury , lead , and other heavy metals . And they are easy to clean and dry.
Boho throw pillows are great for your boho wedding dining , bedding room , sofa , or home bedding decoration as couch pillows , lumbar pillow , floor pillows , chair cushions , bed cushion covers ect .
Macrame Cushions,Macrame Cushion,Cushion Covers,Macrame Cushion Cover
Shandong Guyi Crafts Co.,Ltd , https://www.gyicraft.com