The separation principle of chromatography is: when the components dissolved in the mobile phase (mobile phase) pass through the stationary phase, due to the interaction with the stationary phase (adsorption, distribution, ion attraction, exclusion, affinity) The size and strength are different, and the residence time in the stationary phase is different, so that it flows out from the stationary phase successively. Also called chromatographic method and chromatography.
Chromatography was first discovered by Russian botanist Tswett when he studied the separation of plant pigments with calcium carbonate in 1906. Chromatography was named after it. Later on this basis, paper chromatography, thin layer chromatography, gas chromatography, and liquid chromatography were developed.
The beginning of liquid chromatography is to use a large-diameter glass column to transport the mobile phase with liquid level difference at room temperature and normal pressure. It is called classic liquid chromatography. This method has low column efficiency and long time (often several hours) ). High performance liquid chromatography (High performance Liquid Chromatography, HPLC) is based on the classic liquid chromatography method, and it was developed rapidly in the late 1960s by introducing gas chromatography theory. The difference between it and classic liquid chromatography is that the filler particles are small and uniform. The small particles have high column efficiency, but they will cause high resistance, and the mobile phase needs to be transported with high pressure, so it is also called high pressure liquid chromatography (High Pressure Liquid Chromatography, HPLC) . It is also called High Speed ​​Liquid Chromatography (HSLP) due to its fast analysis speed. Also known as modern liquid chromatography.
Chromatography was first discovered by Russian botanist Tswett when he studied the separation of plant pigments with calcium carbonate in 1906. Chromatography was named after it. Later on this basis, paper chromatography, thin layer chromatography, gas chromatography, and liquid chromatography were developed.
The beginning of liquid chromatography is to use a large-diameter glass column to transport the mobile phase with liquid level difference at room temperature and normal pressure. It is called classic liquid chromatography. This method has low column efficiency and long time (often several hours) ). High performance liquid chromatography (High performance Liquid Chromatography, HPLC) is based on the classic liquid chromatography method, and it was developed rapidly in the late 1960s by introducing gas chromatography theory. The difference between it and classic liquid chromatography is that the filler particles are small and uniform. The small particles have high column efficiency, but they will cause high resistance, and the mobile phase needs to be transported with high pressure, so it is also called high pressure liquid chromatography (High Pressure Liquid Chromatography, HPLC) . It is also called High Speed ​​Liquid Chromatography (HSLP) due to its fast analysis speed. Also known as modern liquid chromatography.
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