Experimental animal immunization program

Using the basic theories and techniques of immunology to diagnose, prevent and treat diseases, and on the basis of this, artificial methods are used to input immune effectors produced by others or animals, such as immune serum and lymphokines, so that the body can be immediately Get immunity. It is characterized by a fast effect and acts immediately after input. However, since the immunity is not produced by the autoimmune system and is easily removed, the immune function is maintained for a short period of time, generally only 2 to 3 weeks. Mainly used for treatment and emergency prevention.

1. Antigen: Protein, polypeptide, organelle, cell, tissue, etc.

2, immune mode: subcutaneous injection, intraperitoneal injection, intravenous injection, intramuscular injection.

3. The amount of antigen required for immunization of different animals (taking protein immunization as an example)

4. Immunization program (taking immune rabbits as an example):

5, pay attention to:

The first immunization was mixed with the immunogen with a complete adjuvant, and then boosted with an incomplete adjuvant mixed with the immunogen, taking a multi-point, time-interval immunoassay.

If the rabbit is immunized with cells, the amount of cells required for each immunization is 2-3 x 107 cells. After three consecutive immunizations, a small amount of blood is taken for ELISA to detect the antibody titer of the immunized animal, and the titer can reach 1:10,000-50,000. One boost should be boosted one week before the immunization of the animals. Shanghai Chuangsai Technology provides mouse vitamin D3Elisa kit, Mouse Vitamin D3, VD3 ELISA Kit, product number: LH-E10203MU-96T, the price is 1530 yuan.

If immunized with mice, a small amount of blood can be collected from the tail vein (about 50 μl), and finally a large amount of blood is collected from the eyeball (about 500-1000 μl); if immunized with a rabbit, a small amount of blood can be taken from the ear vein (about 2 mL), and finally A large amount of blood was taken from the heart (50-100 mL).